LOCUS       40924_49107        4098 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD019, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4098)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4098)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     rep_origin      892..1480
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(1721..1830)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2154..2600)
                     /gene="ndk"
                     /label=ndk
                     /note="Nucleoside diphosphate kinase from Synechococcus 
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805). 
                     Accession#: P50590"
     misc_feature    2606..2611
                     /label=XbaI
                     /note="XbaI"
     misc_feature    2793..2798
                     /label=NheI
                     /note="NheI"
     misc_feature    2853..2858
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    2888..2893
                     /label=NheI
                     /note="NheI"
     misc_feature    2894..2914
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2912..2917
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(3041..3898)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3899..4003)
                     /label=AmpR promoter
     misc_feature    4029..4034
                     /label=ZraI
                     /note="ZraI"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagcgacgt cgggggcccc
       61 cggggggatt ctagattctg gagcaggaag atgtcgcggg cattagcacc agcggtctgc
      121 caagcctccg ccagccgttg ggtcccttcc gcttgagctt ttccatcttc gacgatacgg
      181 gcggcggccc cccgcgcttc cgcgatcgcc cgtttacaag ctgcctcagc tggggcgatc
      241 acatcggctt gaagttgctg ctgcacctgt ttgatccgct cctgctgcac agggagttct
      301 gcttggctac gagcgacttc ggtagcaatg tccgcttcag cttcggccac caccgcttcg
      361 cgccgcgtca acgcatcctg aatccggcgc tcggcctcgg cttgggcgat cgctacatcg
      421 cgatcgatcc gacgcagggc cgtgatcttg tcattttcgg ccgtttggat cgcagaggca
      481 gcctgggcat cggcttcagc aattcgggca tctcgctgca gatcagcccg ctgcttgcgt
      541 ccactagccg agagataacc gacctcatcg gaaatgttct ggacttgcag cgtatcgagg
      601 actagaccca gctgctcaag gtcatcctcc gcctcttcca gcagactttt ggcaaaggca
      661 attttgtcct cgttgatctg ctccggcgtg aggctggcta aaacaccacg caagttgcct
      721 tcgagggtct ccttggcaat ttgctcgatt tccttacggt ttttgccaag cagccgctcg
      781 atcgcgttgt ggatggtcgg ttcttcccca gcaatcttga tattggcaac gccttcaaca
      841 gtcaggggaa tgccgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1021 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gactggttgg cttggtttca
     1681 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     1741 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     1801 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     1861 tctacgcgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     1921 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aagatccctg acgggcttgt
     1981 ctgctcccgg catccgctta cagacaagct ccgcggcgga gcgctgcttt cttggcaagc
     2041 ggtcgccagc cccaacgcca gggctgccag cccgaaacag cggggcaagg cagcttggaa
     2101 gggcgatcgc agcacgggca tggcaatgtc tctctgaagg aatgcagacc ttattcgtac
     2161 agccagggtt gaatcgtggg ggtccaatca cttagctctg ctgggctaaa ccagagagca
     2221 atttcctgtt gtgctgtttc gattgcatcc gagccatgga tgatgttgcg gccaatattg
     2281 acaccaaaat caccacggat ggtgcccggt tctgccgtca gcggattggt agcgccgatc
     2341 aacttgcgag cagccgccac aacgccttcg ccttccaaga cgatcgccac gatcggccca
     2401 gaggtgatga actcgacgag gccattgaag aaggggcgct cgcggtggac agcatagtgc
     2461 tgttcggcca gctcgcgact gggcttcagc tgctttaggc ccaccagttt gaagcctttt
     2521 tgctcaaagc ggccgatgat cgtaccgacc aaaccccgct gaacgccatc gggcttgatg
     2581 gcaataaatg tgcgttccac agacatctag atagtcctca agacgaggca agcattgagc
     2641 ttgccttcct atggttcggg atcactggga ttcttgacaa gcgatcgcgg tcacatcgct
     2701 atctcttagg acttcgcagc gggcgagtcg gattgacccg gtagggattt cgccagatca
     2761 atgcccgtgg tttgtttcag cttctccagc aagctagcga tttgggtagc gctgccttcc
     2821 ccttcgccaa tcacagtgat cgactccacg tcgatatctg gcacggtgcc tgaaagcgtg
     2881 acgagcagct agcatcgcgc gcgcgcgcgc ggacgtcctc acgttaaggg attttggtca
     2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4021 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4081 tcacgaggcc ctttcgtc
//
